PALMA: mRNA to genome alignments using large margin algorithms

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PALMA: mRNA to genome alignments using large margin algorithms

MOTIVATION Despite many years of research on how to properly align sequences in the presence of sequencing errors, alternative splicing and micro-exons, the correct alignment of mRNA sequences to genomic DNA is still a challenging task. RESULTS We present a novel approach based on large margin learning that combines accurate splice site predictions with common sequence alignment techniques. B...

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PALMA: Perfect Alignments using Large Margin Algorithms

Despite many years of research on how to properly align sequences in the presence of sequencing errors, alternative splicing and micro-exons, the correct alignment of mRNA sequences to genomic DNA is still a challenging task. We present a novel approach based on large margin learning that combines kernel based splice site predictions with common sequence alignment techniques. By solving a conve...

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Guanidine hydrochloride has been widely used in the initial recovery steps of active protein from the inclusion bodies in aqueous two-phase system (ATPS). The knowledge of the guanidine hydrochloride effects on the liquid-liquid equilibrium (LLE) phase diagram behavior is still inadequate and no comprehensive theory exists for the prediction of the experimental trends. Therefore the effect the ...

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Advances in sequencing technologies have substantially increased the number of fully sequenced genomes. Alignment algorithms play a crucial rule in analyzing whole genomes, identifying similar and conserved regions between pairs of genomes, leading to annotation of genomes with site-specific properties and functions. In this work we introduce a parallel algorithm for a widely used whole genome ...

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Multiple alignments among genomes are becoming increasingly prevalent. This trend motivates the development of tools for efficient homology search between a query sequence and a database of multiple alignments. In this paper, we present an algorithm that uses the information implicit in a multiple alignment to dynamically build an index that is weighted most heavily towards the promising region...

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ژورنال

عنوان ژورنال: Bioinformatics

سال: 2007

ISSN: 1367-4803,1460-2059

DOI: 10.1093/bioinformatics/btm275